山羊骨骼肌中miR-133及其靶基因的表达规律与功能研究

doi:10.11843/j.issn.0366-6964.2015.11.005

Abstract

Abstract:

The purpose of this experiment was to study the expression of miR-133 and its target gene SRF in skeletal muscle tissue and cells in goat，and the effects of miR-133 on target gene（SRF）.The qRT-PCR was used to detect different expression of miR-133 and its target gene in different tissues of newborn，7 months and 18 months old Anhui white goat and Boer goat（heart，liver，spleen，lung，kidney，small intestine，fat，longissimus dorsi） and different goat skeletal muscle satellite cells（F6，F9，F12）；qRT-PCR and the Western blot was used to detect the mRNA of target gene and protein expression level.The results of qRT-PCR showed that the expression level of miR-133 in myocardium and longissimus dorsi muscle was higher than that in other tissues，the expression level of the target gene SRF was different from miR-133 in goat；The expression level of miR-133 in skeletal muscle satellite cells increased with the cell generations；That regulation of miR-133 on SRF protein expression occurred at the post transcriptional level by qRT-PCR and Western blot.The results showed that miR-133 was expected to become a new marker of goat skeletal muscle growth and development；miR-133 was involved in the regulation of proliferation and differentiation of goat by inhibiting the expression of SRF protein in the post transcriptional level，thereby affecting the goat skeletal muscle development.

CLC Number:

S827.2

LING Ying-hui，WANG Li-juan，WANG Kang-yan，LI Yun-sheng，DING Jian-ping，ZHANG Yun-hai，ZHANG Xiao-rong. The Expression Profiles and Function Analysis of miR-133 in Goat Skeletal Muscle Tissue and Cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1944-1951.

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The Expression Profiles and Function Analysis of miR-133 in Goat Skeletal Muscle Tissue and Cell

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